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Qualification of Spectrophotometers

UV/Vis spectrophotometry is one of the most frequently applied methods in chemical analysis. It is used for example in clinical chemistry, pharmaceutical industry, scientific research and very often in quality assurance.

In recent years quality requirements, as outlined by EN ISO 9001/17025, Good Laboratory Practice (GLP), Good Manufacturing Practice (GMP) or the recommendations of pharmacopeias (EP, DAB, USP) have become more influential. Formal performance verification of UV/Vis spectrophotometers is now essential.

    The performance verification tests required by major Pharmacopeias for UV/Vis spectrophotometers are the following:
  • Photometric accuracy
  • Wavelength accuracy
  • Stray light
  • Spectral resolution

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